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mammalian expression vector pegfp c2  (Addgene inc)


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    Structured Review

    Addgene inc mammalian expression vector pegfp c2
    ( a ) reports representative dot plots obtained evaluating GFP positive cells by flow cytometry after NF of a <t>pEGFP</t> <t>C2</t> vector encoding for CD63 in 10% FBS and 5% SRGF by C17 program. The gating threshold (vertical solid line) identifying GFP negative cells was defined analyzing ASC after NF without DNA vector (as control). ( b ) reports the evaluation by Western blot analysis of CD63 overexpression after NF by C-17 program of a pEGFP C2 vector encoding for CD63. For Western blot analysis, a pEGFP C2 empty vector was transfected (as control condition) in both 10% FBS and 5% SRGF by C17 program. Original unmodified images of analyzed Western blot are reported in . CD63 band density quantification was expressed as percent ratio to the housekeeping gene GAPDH. *; p < 0.01 vs. 10% FBS ASC. Reported images and quantification values represent results derived from at least three independent experimental tests.
    Mammalian Expression Vector Pegfp C2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 91 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mammalian+expression+vector+pegfp+c2/pmc08700287-74-9-14?v=Addgene+inc
    Average 95 stars, based on 91 article reviews
    mammalian expression vector pegfp c2 - by Bioz Stars, 2026-07
    95/100 stars

    Images

    1) Product Images from "Nucleofection of Adipose Mesenchymal Stem/Stromal Cells: Improved Transfection Efficiency for GMP Grade Applications"

    Article Title: Nucleofection of Adipose Mesenchymal Stem/Stromal Cells: Improved Transfection Efficiency for GMP Grade Applications

    Journal: Cells

    doi: 10.3390/cells10123412

    ( a ) reports representative dot plots obtained evaluating GFP positive cells by flow cytometry after NF of a pEGFP C2 vector encoding for CD63 in 10% FBS and 5% SRGF by C17 program. The gating threshold (vertical solid line) identifying GFP negative cells was defined analyzing ASC after NF without DNA vector (as control). ( b ) reports the evaluation by Western blot analysis of CD63 overexpression after NF by C-17 program of a pEGFP C2 vector encoding for CD63. For Western blot analysis, a pEGFP C2 empty vector was transfected (as control condition) in both 10% FBS and 5% SRGF by C17 program. Original unmodified images of analyzed Western blot are reported in . CD63 band density quantification was expressed as percent ratio to the housekeeping gene GAPDH. *; p < 0.01 vs. 10% FBS ASC. Reported images and quantification values represent results derived from at least three independent experimental tests.
    Figure Legend Snippet: ( a ) reports representative dot plots obtained evaluating GFP positive cells by flow cytometry after NF of a pEGFP C2 vector encoding for CD63 in 10% FBS and 5% SRGF by C17 program. The gating threshold (vertical solid line) identifying GFP negative cells was defined analyzing ASC after NF without DNA vector (as control). ( b ) reports the evaluation by Western blot analysis of CD63 overexpression after NF by C-17 program of a pEGFP C2 vector encoding for CD63. For Western blot analysis, a pEGFP C2 empty vector was transfected (as control condition) in both 10% FBS and 5% SRGF by C17 program. Original unmodified images of analyzed Western blot are reported in . CD63 band density quantification was expressed as percent ratio to the housekeeping gene GAPDH. *; p < 0.01 vs. 10% FBS ASC. Reported images and quantification values represent results derived from at least three independent experimental tests.

    Techniques Used: Flow Cytometry, Plasmid Preparation, Control, Western Blot, Over Expression, Transfection, Derivative Assay



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    Image Search Results


    ( a ) reports representative dot plots obtained evaluating GFP positive cells by flow cytometry after NF of a pEGFP C2 vector encoding for CD63 in 10% FBS and 5% SRGF by C17 program. The gating threshold (vertical solid line) identifying GFP negative cells was defined analyzing ASC after NF without DNA vector (as control). ( b ) reports the evaluation by Western blot analysis of CD63 overexpression after NF by C-17 program of a pEGFP C2 vector encoding for CD63. For Western blot analysis, a pEGFP C2 empty vector was transfected (as control condition) in both 10% FBS and 5% SRGF by C17 program. Original unmodified images of analyzed Western blot are reported in . CD63 band density quantification was expressed as percent ratio to the housekeeping gene GAPDH. *; p < 0.01 vs. 10% FBS ASC. Reported images and quantification values represent results derived from at least three independent experimental tests.

    Journal: Cells

    Article Title: Nucleofection of Adipose Mesenchymal Stem/Stromal Cells: Improved Transfection Efficiency for GMP Grade Applications

    doi: 10.3390/cells10123412

    Figure Lengend Snippet: ( a ) reports representative dot plots obtained evaluating GFP positive cells by flow cytometry after NF of a pEGFP C2 vector encoding for CD63 in 10% FBS and 5% SRGF by C17 program. The gating threshold (vertical solid line) identifying GFP negative cells was defined analyzing ASC after NF without DNA vector (as control). ( b ) reports the evaluation by Western blot analysis of CD63 overexpression after NF by C-17 program of a pEGFP C2 vector encoding for CD63. For Western blot analysis, a pEGFP C2 empty vector was transfected (as control condition) in both 10% FBS and 5% SRGF by C17 program. Original unmodified images of analyzed Western blot are reported in . CD63 band density quantification was expressed as percent ratio to the housekeeping gene GAPDH. *; p < 0.01 vs. 10% FBS ASC. Reported images and quantification values represent results derived from at least three independent experimental tests.

    Article Snippet: In such experiments, NF of 1 μg of the mammalian expression vector pEGFP C2 (AddGene) containing the CD63 gene sequence was performed.

    Techniques: Flow Cytometry, Plasmid Preparation, Control, Western Blot, Over Expression, Transfection, Derivative Assay